Publikationen der AG Zellkulturtechnik seit 2005

Peer reviewed Journals


Romanova N and Noll T (2018), Engineered and natural promoters and chromatin-modifying elements for recombinant protein expression in CHO cells, Biotechnol J, doi: 10.1002/biot.201700232 




Wippermann A, Rupp O, Brinkrolf K, Hoffrogge R, Noll T (2017), Integrative analysis of DNA methylation and gene expression in butyrate-treated CHO cells, J Biotechnol, 257: 150-61


Müller B, Heinrich C, Jabs W, Kaspar-Schönefeld S, Schmidt A, Rodriguez de Carvalho N, Albaum SP, Baessmann C, Noll T, Hoffrogge R (2017), Label-free protein quantification of sodium butyrate treated CHO cells by ESI-UHR-ToF-MS, J Biotechnol, 257: 87-98


Wippermann A, Noll T (2017), DNA methylation in CHO cells, J Biotechnol, 258: 206-210




Beatson R, Tajadura-Ortega V, Achkova D, Picco G, Tsourouktsoglou TD, Klausing S, Hillier M, Maher J, Noll T, Crocker PR, Taylor-Papadimitriou J, Burchell JM (2016), MUC1 modulates the tumor immune microenvironment through the engagement of Siglec-9, Nature Immunology, 17(11): 1273-81 


Coronel J, Klausing S, Heinrich C, Noll T, Figueredo-Cardero A, Castilho LR (2016), Valeric acid supplementation combined to mild hypothermia increases productivity in CHO cell cultivations, Biochemical Engineering Journal, 114:101-109



Brandenbusch C, Glonke S, Collins J, Hoffrogge R, Grunwald K, Bühler B, Schmid A, Sadowski G (2015), Process Boundaries of Irreversible scCO2-assisted Phase Separation in Bi-Phasic Whole Cell Biocatalysis, Biotechnol Bioeng, doi: 10.1002/bit.25655


Wippermann A, Brinkrolf K, Rupp O, Hoffrogge R, Noll T (2015), The DNA methylation landscape of Chinese Hamster Ovary (CHO) DP-12 cells, J Biotechnol, 199:38-46


Wingens M, Gaetgens J, Schmidt A, Albaum SP, Buentemeyer H, Noll T, Hoffrogge R (2015), 2D-DIGE screening of high-productive CHO cells under glucose limitation - Basic changes in the proteome equipment and hints for epigenetic effects, J Biotechnol, 201:86-97



Jakobi T, Brinkrolf K, Tauch A, Noll T, Stoye J, Puehler A, Goesmann A (2014), Discovery of transcription start sites in the Chinese hamster genome by next-generation RNA sequencing, J Biotechnol 190:64-75


Lohr V, Hädicke O, Genzel Y, Jordan I, Büntemeyer H, Klamt S, Reichl U (2014), The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis, BMC Biotechnology 2014, 14:72


Noll T, Puehler A, Weisshaar B, Wendisch VF (2014), The genomics revolution and its impact on future biotechnology, J Biotechnol 190:1


Wippermann A, Klausing S, Rupp O, Albaum SP, Buentemeyer H, Noll T, Hoffrogge R (2014), Establishment of a CpG island microarray for analyses of genome-wide DNA methylation in Chinese hamster ovary cells, Appl Microbiol Biotechnol 98(2): 579-89


Rath AG, Rehberg M, Janke R, Genzel Y, Scholz S, Noll T, Rose T, Sandig V, Reichl U (2014), The influence of cell growth and enzyme activity changes on intracellular metabolite dynamics in AGE1.HN.AAT cells, J Biotechnol 178: 43-53


Becker J, Timmermann C, Klausing S, Rupp O, Brinkrolf K, Goesmann A, Tauch A, Noll T (2014),Transcriptome analyses of CHO cells with the next-generation microarray CHO41K:development and validation by analysing the influence of the growth stimulating substance IGF-1 substitute LongR3, J Biotechnol 178:23-31


Rupp O, Becker J, Brinkolf K, Timmermann C, Borth N, Pühler A, Noll T, Goesmann A (2014), Construction of a public CHO celline transcipt database using versatile bioinformatics analysis pipelines, PLOS ONE, 9(1): e85568. doi:10.1371/journal.pone.0085568



Klenke C, Widera D, Engelen T, Müller J, Noll T, Niehaus K, Schmitz ML, Kaltschmidt B, Kaltschmidt C (2013) Hsc70 Is a Novel Interactor of NF-kappaB p65 in Living Hippocampal Neurons, PLOS ONE 8(6): e65280


Brinkrolf K, Rupp O, Laux H, Kollin F, Ernst W, Linke B, Kofler R, Romand S, Hesse F, Budach WE, Galosy S, Müller D, Noll T, Wienberg J, Jostock T, Leonard M, Grillari J, Tauch A, Goesmann A, Helk B, Mott JE, Pühler A and Borth N (2013), Chinese hamster genome sequenced from sorted chromosomes, Nature Biotechnology, 31(8): 694-5


Wippermann A, Klausing S, Rupp O, Albaum SP, Büntemeyer H, Noll T, Hoffrogge R (2013), Establishment of a CpG island microarray for analyses of genome-wide DNA methylation in Chinese hamster ovary cells, Appl Microbiol Biotechnol, DOI 10.1007/s00253-013-5282-2    [download]


Freund S, Rath A, Platas Barradas O, Skerhutt E, Scholz S, Niklas J, SandigV, Rose T, Heinzle E, Noll T, Pörtner R, Zeng AP, Reichl R (2013), Batch-to-batch variability of two human designer cell lines AGE1.HN and AGE1.HN.AAT carried out by different laboratories under defined culture conditions using a mathematical model, Engineering in Life Sciences 03/2013; DOI:10.1002/elsc.201200111



Platas Barradas O, Jandt U, Da Minh Phan L, Villanueva ME, Schaletzky M, Rath A, Freund S, Reichl U, Skerhutt E, Scholz S, Noll T, Sandig V, Pörtner R, Zeng AP (2012), Evaluation of criteria for bioreactor comparison and operation  standardization for mammalian cell culture, Eng. Life Sci. 12(5): 1–11


Carrondo M, Alves P, Carinhas N, Glassey J, Hesse F, Merten OW, Micheletti M, Noll T, Freitas Oliveira RM, Reichl U, Staby A, Teixeira A, Weichert H, Mandenius CF (2012), How can measurement, monitoring, modelling and control advance cell culture in industrial biotechnology?, Biotechnol J, accepted


Hackl M, Jadhav V, Jakobi T, Rupp O, Brinkrolf K, Goesmann A, Pühler A, Noll T, Borth N, Grillari J (2012) Computational identification of microRNA gene loci and precursor microRNA sequences in CHO cell lines, J Biotechnol, 158(3): 151-5


Meleady P, Hoffrogge R, Henry M, Rupp O, Bort JH, Clarke C, Brinkrolf K, Kelly S, Müller B, Doolan P, Hackl M, Beckmann T, Noll T, Grillari J, Barron N, Pühler A, Clynes M, Borth N (2012), Utilisation and evaluation of CHO-specific sequence databases for mass spectrometry based proteomics, Biotechnol Bioeng, 109(6): 1386-94


Beckmann TF, Krämer O, Klausing S, Heinrich C, Thüte T, Büntemeyer H, Hoffrogge R, Noll T (2012), Effects of high passage cultivation on CHO cells: a global analysis, Appl. Microbiol Biotechnol, 94:659-71



 Becker J., Hackl M, Jakobi T, Rupp O, Schneider J, Szczepanowski R, Bekel T, Borth N, Goesmann A, Grillari J, Kaltschmidt C, Noll T, Pühler A, Tauch A, Brinkrolf K (2011), Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing, J Biotechnology, 156(3): 227-35


Volmer M, Northoff S, Scholz S, Thüte T, Büntemeyer H, Noll T (2011) Fast Filtration for Metabolome Sampling of Suspended Animal Cells, Biotechnology Letters, 33(3):495-502


Niklas J, Schräder E, Sandig V, Noll T, Heinzle E (2011) Quantitative characterization of metabolism and metabolic shifts during growth of the new human cell line AGE1.HN using time resolved metabolic flux analysis, Bioprocess Biosystems Engineering, 34(5):533-45


Lenger J, Schröder M, Ennemann E, Müller B, Wong CH, Noll T, Dierks T, Hanson SR and Sewald N, (2011), Evaluation of Sulfatase-Directed Quinone Methide Traps for Proteomics, Bioorg Med Chem, 20:622-27



Krämer O, Klausing S, Noll T (2010) Methods in mammalian cell line engineering: from random mutagenesis to sequence-specific approaches, Applied Microbiology and Biotechnology, 88(2):425-436


Burgemeister S, Nattkemper T, Noll T, Hoffrogge R, Flaschel E (2010) CellViCAM - Cell Viability Classification for Animal Cell Cultures using Dark Field Micrographs, Journal of Biotechnology, 149(4):310-316



Kubitzki T, Minör D, Mackfeld U, Noll T, Oldiges M, Lütz S (2009) Application of immobilized bovine enterokinase in repetitive fusion protein cleavage for the production of MUC1, Biotechnology Journal, 4(11):1610-8


Batista FRX, Ângela M. Moraes AM, Büntemeyer H, Noll T (2009) Influence of Culture Conditions on Recombinant Drosophila melanogaster S2 Cells Producing the G Glycoprotein from the Surface of Rabies Virus Cultivated in Serum-Free Medium, Biologics, 2009, 37:108


Widera D, Zander C, Heidbreder M, Kasperek Y,  Noll T, Seitz O, Saldamli B, Sudhoff H, Sader H, Kaltschmidt C and Kaltschmidt B (2009) Adult palatum as a novel source of neural-crest related stem cells, Stem Cells, Stem Cells 27(8):1899-910


Sihlbom C, van Dijk Härd I, Lidell ME, Noll T, Hansson GC, Bäckström M (2009) Localisation of O-glycans in MUC1 glycoproteins using electron-capture dissociation fragmentation mass spectrometry, Glycobiology, 19(4):375-81


Magin AS, Körfer N, Partenheimer H, Lange C, Zander A, Noll T (2009) Primary cells as feeder cells for clinical co-culture expansion of human hematopoietic stem cells from umbilical cord blood – a comparative study, Stem Cell and Development 8(1):173-86



Kubitzki T, Noll T, Lütz S (2008) Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage, Bioprocess Biosyst Eng. 2008, 3:173-82


Grimshaw MJ, Papazisis K, Picco G, Bohnenkamp HR, Noll T, Taylor-Papadimitriou J, Burchell J (2008) Immunisation with ‘naïve’ syngeneic dendritic cells protects mice from tumour challenge, Br J Cancer. 98(4):784-91


Schweppe CH, Bielaszewska M, Pohlentz G, Friedrich AW, Büntemeyer H, Schmidt MA, Kim KS, Peter-Katalinic J, Karch H, Müthing J (2008) Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with different Shiga toxin 1 cytotoxicity, Glycoconj J,  25:291-304


Niebruegge S, Nehring A, Bär H, Schroeder M, Zweigerdt R, Lehmann J (2008), Cardiomyocyte production in mass suspension culture: embryonic stem cells as a source for great amounts of functional cardiomyocytes, Tissue Eng Part A, 14(10):1591-601



Wiendahl C, Brandner J, Küppers C, Luo B, Schygulla U, Noll T, Oldiges M (2007), A microstructure heat exchanger for quenching the metabolism of mammalian cells, Chem Eng Technol, 30(3): 322-28


Hoffrogge R., Beyer S., Hubner R., Mikkat S., Mix E., Scharf C., Schmitz U., Pauleweit S., Berth M., Zubrzycki I.Z., Christoph H., Pahnke J., Wolkenhauer O., Uhrmacher A., Volker U., Rolfs A. (2007), 2-DE profiling of GDNF overexpression-related proteome changes in differentiating ST14A rat progenitor cells. Proteomics: 7(1): 33-46


Beyer S, Mix E, Hoffrogge R, Lünser K, Völker U, Rolfs A (2007), Neuroproteomics in stem cell differentiation. Clinical Proteomics 1 (11): 1513 - 1523



Hoffrogge R., Mikkat S., Scharf C., Christoph H., Beyer S., Pahnke J., Mix E., Berth M., Uhrmacher A., Zubrycki I., Miljan E., Völker U., Rolfs A. (2006), 2-DE proteome analysis of a proliferating and differentiating human neuronal stem cell line (ReNcell VM). Proteomics: 6(6): 1833-47


Hoffrogge R., Beyer S., Völker U., Uhrmacher A., Rolfs A. (2006), 2-DE proteomic profiling of neuronal stem cells. Neurodegenerative Dis 3: 112-121


Mix E., Ibrahim S.M., Pahnke J., Glass A., Mazon-Pelaez I., Lemcke S., Koczan D., Gimsa U., Bansemer S., Scheel T., Karopka T., Bottcher T., Muller J., Dazert E., Antipova V., Hoffrogge R., Wree A., Zschiesche M., Strauss U., Kundt G., Warzok R., Gierl L., Rolfs A. (2006), 3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitor Atorvastatin mediated effects depend on the activation status of target cells in PLP-EAE. J Autoimmun. 27(4):251-65


Guthke R, zeilinger K, Sickinger S, Schmidt-Heck W, Büntemeyer H, Iding K, Lehmann J, Pfaff M, Pless G, Gerlach JC (2006), Dynamics of amino acid metabolism of primary human liver cells in 3D bioreactors, Bioprocess Biosys Eng, 28: 331-340


Sewell R, Dalziel M, Backstrom M, Hansson G, Noll T, Clausen H, Burchell J, Taylor-Papadimitriou J (2006) Human ST6GalNAcI is responsible for the expression of the sialyl-Tn O-glycan in breast cancers, J Biol Chem, 281:3586-94



Bohnenkamp HR, Burchell J, Taylor-Papadimitriou J, Noll T (2005) Apoptosis of monocytes and the influence on yield of monocyte-derived dendritic cells, Journal of Immunol Methods, 294(1-2):67-80.


Rughetti A, Pellicciotta I, Biffoni M, Bäckström M, Link T, Noll T, Hansson GC, Clausen H, Burchell JM, Taylor-Papadimitriou J, Nuti M (2005) Recombinant   tumor associated   MUC1  impairs differentiation  and function of Dendritic Cells, Journal of Immunology, 174(12):7764-72    


Markotic A, Culic VC, Kurir TT, Meisen I, Büntemeyer HBoraska V, Zemunik T, Petri N, Mesaric M, Peter-Katalinic J, müthing J (2005), Oxygenation alters ganglioside expression in rat liver following partial hepatectomy, Biochem Biophys Res Commun, 330: 131-141


Schroeder M, Niebruegge S, Werner A, Willbold E, Burg M, Ruediger M, Field LJ, Lehmann J, Zweigerdt R (2005), Differentiation and lineage selection of mouse embryonic stem cells in a stirred bench scale bioreactor with automated process control, Biotechnol Bioeng, 92(7):920-33




Wippermann A, Klausing S, Rupp O, Noll T, Hoffrogge R (2013), First CpG island micrarray for genome-wide analyses o DNA methylation i chinese hamster ovary cells: new insights into the epigenetic answer to butyrate treatment, BMC Proceedings 2013, 7(Suppl 6):O5


Gettmann J, Timermann C, Becker J, Thüte T, Rupp O, Büntemeyer H, Lohmeier A, Scholz S, Goesmann A (2013) Multidimension cultivation analysis by standard and omics methods for optimization of therapeutics production, BMC Proceedings 2013, 7(Suppl 6):P5


Lohmeier A, Northoff S, Munro T, Noll T (2013) Effects of perfusion processes under limiting conditions on different Chinese Hamster Ovary cells, BMC Proceedings 2013, 7(Suppl 6):P64


Klausing S, Krämer O, Noll T (2013) Enhancing cell growth and antibody production in CHO cells by siRNA knockdown of novel target genes, BMC Proceedings 2013, 7(Suppl 6):P92


Aretz J, Thüte T, Scholz S, Kersting K, Noll T, Büntemeyer H (2013) Understanding cell behavior in cultivation processes - A metabolic approach, BMC Proceedings 2013, 7(Suppl 6):P90



Skerhutt EM, Scholz S, Sandig V, Hoffrogge R, Noll T (2011) Analysis of the mitochondrial subproteome of the human cell line AGE1.HN–a contribution to a systems biology approach. BMC Proceedings, 5 (8)

Skerhutt EM, Scholz S, Niklas J, Rath A, Platas Barradas O, Jandt U, Sandig V, Rose T, Pörtner R, Reichl U, Zeng A-P, Heinzle E, Noll T (2011) Characterisation of cultivation of the human cell line AGE1.HN.AAT. BMC Proceedings, 5 (8)

Scholz S, Miriam L, Alexander R, Schräder EM, Thomas R, Büntemeyer H, Thomas S, Udo R, Noll T (2011) Characterization of the human AGE1.HN cell line: a systems biology approach. BMC Proceedings, 5 (8)


Platas Baradas O, Jandt U, Da Minh Phan L, Villanueva M, Rath A , Reichl U, Schräder EM, Scholz S, Noll T, Sandig V, Pörtner R, Zeng AP (2011) Criteria for bioreactor comparison and operation standardisation during process development for mammalian cell culture. BMC Proceedings, 5 (8)

Klausing S, Krämer O, Noll T (2011) Bioreactor cultivation of DP-12 cells under sodium butyrate treatment–comparative transcriptome analysis with CHO cDNA microarrays. BMC Proceedings, 5 (8)

Volmer M, Gettmann J, Scholz S, Büntemeyer H, Noll T (2011) A method for metabolomic sampling of suspended animal cells using fast filtration. BMC Proceedings, 5 (8)


Beckmann T, Thüte T, Heinrich C, Büntemeyer H, Noll T (2011) Proteomic and metabolomic characterization of DP-12 cell lines with different high passage histories. BMC Proceedings, 5 (8)

Heinrich C, Timo W, Christina K, Northoff S, Noll T (2011) Growth characterization of CHO DP-12 cell lines with different high passage histories. BMC Proceedings, 5 (8)

Becker J, Timmermann C, Jakobi T, Rupp O, Szczepanowski R, Hackl M, Goesmann A, Tauch A, Borth N, Grillari J, Pühler A, Noll T, Brinkrolf K (2011) Next-generation sequencing of the CHO cell transcriptome. BMC Proceedings, 5 (8)



Nottorf T, Büntemeyer H, Siwiora-Brenke S, Lütkemeyer D, Loa A, Noll T, Lehmann J (2010), An integrated production process for human growth hormone, Noll (ed.), ‘Cells & Culture’, Springer, 205-8


Wingens M, Gätgens J, Hoffrogge R, Noll T (2010), Proteomic characterization of a glucose-limited CHO perfusion process-analysis of metabolic changes and increase in productivity, Noll (ed.), ‘Cells & Culture’, Springer, 265-70


Bäckström M, Karlsson H, Gätgens J, Noll T, Hansson GC (2010), O-glycans on recombinant MUC1 produced in CHO K1 cells becomes less sialylated with increased productivity as determined by LC ESI MS, Noll (ed.), ‘Cells & Culture’, Springer, 285-8


Wiendahl C, Brandner JJ, Klein B, Küppers C, Luo B, Ritchie S, Mazurek S, Scheefers H, Noll T, Schygulla U, Oldiges M (2010), Metabolome analysis in mammalian cells: Development and application of a sampling technique, Noll (ed.), Cells & Culture, Springer, 413-20


Northoff S, Sandig V, Zietze S, Büntemeyer H, Noll T (2010), Characterization of the novel human AGE1hn cell line for production of recombinant proteins, Noll (ed.), ‘Cells & Culture’, Springer, 693-8



Nottorf T, Hoera W, Büntemeyer H, Siwiora-Brenke S, Loa A, Lehmann J (2007), Production of human growth hormone in a mammalian cell high density perfusion process, Smith (ed) ‘Cell Technology for Cell Products’, Springer, Dordrecht, 789-793


Schaper J, Bohnenkamp HR, Noll T (2007), New electrofusion devices for the improved generation of dendritic cell-tumour cell hybrids, Smith (ed) ‘Cell Technology for Cell Products’, Springer, 207-216


Lamlé J, Büntemeyer H, Reinel A, Menne T, van den Bos C, Lehmann J (2005), Propagation of USSC for theraoeutic approaches, Smith (ed) ‘Cell Technology for Cell Products’, Springer, 325-329


Bauwens A, Büntemeyer H, Terstegge S, Nottorf T, Brüstle O, Lehmann J (2007), Bioreactor based production of functional conditioned medium for the propagation of undifferentiated human embryonic stem cells, Smith (ed) ‘Cell Technology for Cell Products’, Springer, 331-334


Jockwer A, Klinger C, Gätgens J, Eisenkrätzer D, Noll T (2007), Towards pCO2-optimized fermentations – reliable sampling, pCO2 control & cellular metabolism, Smith (ed) ‘Cell Technology for Cell Products’, Springer, 509-518


Schomberg M, Hakemeyer C, Buentemeyer H, Stiens L, Lehmann J (2007), Growth promoting effect of a hydrolysate primatone on hybridoma cells (2007), Smith (ed) `Cell Technology for Cell Products´, Springer, 141 - 146


Niebruegge S, Ebelt H, Jungblut M, Baer H, Schroeder M, Zweigerdt R, Braun T, Lehmann J, (2007), `Generation of Cardiomyocytes derived from mouse ESC in a Scale-up System, Smith (ed) `Cell Technology for Cell Products´, Springer, 191 – 195


Nottorf T, Hoera W, Buentemeyer, Siwiora-Brenke S, Loa A, Lehmann J, (2007), Production of human growth hormone in a mammalian cell high density perfusion process, `Cell Technology for Cell Products´, Springer, 789 – 793



Link T, Essers R, Zörner K, de Graaf A, Bäckström M, Hansson G, Noll T (2005) Development of metabolically optimized fermentation process based on glucose-limited CHO perfusion culture, Godia (ed.) ‘Animal Cell Technology meets Genomics’, Kluwer Academic Publishers, Dordrecht, 423-30  


Fischbach, Noll T (2005) Membrane-separated cocultivation of cord blood hematopoietic stem cells with stromal cell lines, Godia (ed.) ‘Animal Cell Technology meets Genomics’, Kluwer Academic Publishers, 269-71


Bohnenkamp HR, Noll T (2005) Process development for standardized generation of monocyte-derived dendritic cells: Applicability in breast cancer immunotherapy, Godia (ed.) ‘Animal Cell Technology meets Genomics’, Kluwer Academic Publishers, 221-27


Schomberg M, Hakemeyer C, Büntemeyer C, Stiens L, Lehmann J (2005), Growth promoting effect of a hydrophilic fraction of the protein hydrophilic fraction of the protein hydrolysate Primatone on hybridoma cells, Smith (ed) ‘Cell Technology for Cell Products’, Springer, Dordrecht, 141-146


Iding K, Buentemeyer H, Lehmann, J (2005), Non-freezing preservation and subsequent recovery of hepatocytes uner pO2 – and pH-controlled conditions for continuous suppy of cells for bioartificial liver support, Gòdia, Fussenegger (eds) `Animal Cell Technology meets Genomics´, Springer, 167 – 170


Schroeder M, Niebruegge S, Werner A, Zweigerdt R, Burg M, Lehmann J, (2005), Optimizing the production of cardiomycytes from mouse embryonic stem cells in a 2L stirred tank reactor,Gòdia, Fussenegger (eds) `Animal Cell Technology meets Genomics´, Springer, 285 – 288